LOCUS       sequence-118416-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes an inert blue fluorescent protein for use in oxidizing 
            cellular environments including the periplasm and mammalian 
            secretory pathway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Costantini LM, Subach OM, Jaureguiberry-bravo M, Verkhusha VV, Snapp
            EL
  TITLE     Cysteineless non-glycosylated monomeric blue fluorescent protein, 
            secBFP2, for studies in the eukaryotic secretory pathway.
  JOURNAL   Biochem Biophys Res Commun. 2013 Jan 18;430(3):1114-9. doi: 
            10.1016/j.bbrc.2012.12.028. Epub 2012 Dec 19.
  PUBMED    23257162
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2012.12.028"; journalName: "Biochem Biophys Res 
            Commun"; date: "2013-01-18- 18"; volume: "430"; issue: "3"; pages: 
            "1114-9"
COMMENT     Sequence Label: psecBFP2
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..716)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="ELIKENMHMKLYMEGTVDNHHFKATSEGEGKPYEGTQTMRIKVVE
                     GGPLPFAFDILATSFLYGSKTFIDHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQ
                     DTSLQDGSLIYNVKIRGVDFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLV
                     GGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANDETYVEQHEVAVARYSDL
                     PSKLGHKLN"
     misc_feature    739..795
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 tgatctagag tcgcggccgc ttaattaagc ttgtgcccca gtttgctagg gaggtcgctg
       61 tatctggcca ctgccacctc gtgctgctcg acgtaggtct cgtcgttggc ctccttgatt
      121 ctttccagtc tgtagtccac atagtagacg ccaggcatct tgaggttctt agcgggtttc
      181 ttggatctat atgtggtctt ggcgtttgcg atcagatggc tcccgcccac gagcttcagg
      241 gccatgtcgt ttctgccttc caggccgccg tcagcggggt acagcgtctc ggtgaaggcc
      301 tcccagccga gtgttttctt ctgcatcaca gggccgttgg atgtgaagtc cacccctctg
      361 atcttgacgt tgtagatgag gctgccgtcc tggaggctgg tgtcctgggt agcggtcagc
      421 acgcccccgt cttcgtatgt ggtgactctc tcccatgtga agccctcagg gaaggactgc
      481 ttgaagaagt cggggatgcc ctgggtgtgg tcgatgaagg tcttgctgcc gtagaggaag
      541 ctagtagcca ggatgtcgaa ggcgaagggg agagggccgc cctcgaccac cttgattctc
      601 atggtctggg tgccctcgta gggcttgcct tcgccctcgg atgttgcctt gaagtgatgg
      661 ttgtccacgg tgccctccat gtacagcttc atgtgcatgt tctccttaat cagctcctcg
      721 ctcatggtgg cgaccggtgg atcccgggcc cgcggtaccg tcgactgcag aattcgaagc
      781 ttgagctcga gatctgag
//
