LOCUS       sequence-118417-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual expression of GBD-LUMP and cdc42.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Hoepker AC, Wang A, Le Marois A, Suhling K, Yan Y, Marriott G
  TITLE     Genetically encoded sensors of protein hydrodynamics and molecular 
            proximity.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 30. pii: 201424021.
  PUBMED    25931526
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Apr 30. pii: 201424021."
COMMENT     Sequence Label: 213-petDuet1-GBD-LUMP--cdc42
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    68..92
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             107..129
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cgatgcgtcc ggcgtagagg atcgagatcg atctcgatcc cgcgaaatta atacgactca
       61 ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg tttaacttta
      121 agaaggagat ataccatggg cctgagcgca caggacatca gccagccgct gcagaacagc
      181 ttcatccata ccggccacgg cgacagcgat cctcgccatt gctggggctt tccggaccgc
      241 atcgacgagc tgtacctggg caacggcagc ggcagcgcta gctttagagg tattgttcaa
      301 ggtcgtggtg ttattcgatc catttcaaaa agtgaagata gtcagcgaca tgggattgca
      361 ttccctgagg gtatgtttca acttgttgat gtagatactg taatgttagt aaatggttgt
      421 tcattgacag ttgtccgtat tttaggcgat atggtgtact ttgatatcga ccaagcattg
      481 ggtacgacaa catttgatgg attaaaagaa ggcgatcagg taaacctaga gatccaccct
      541 aaatttggtg aagtggttgg ccgtggtgga ttaacaggaa atattaaagg cactgcgctg
      601 gtcgcagcaa tagaagagaa tgacgcaggt ttttctgtac tgattgatat ccctaaaggg
      661 ttagctgaaa acttaacagt gaaagatgat attggaattg atgggatctc tttgcctatc
      721 actgatatgt cagacagcat tattacattg aattactcgc gtgatttgtt agcgagtact
      781 aacattgcat cgttagcaaa agatgtgaaa gtgaacgttg aaattttaaa tgaatggtag
      841 gcggccgcac tcaagcttgc ggccgcataa tgctta
//
