LOCUS       sequence-118419-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Myc-tagged LATS1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Bao Y, Nakagawa K, Yang Z, Ikeda M, Withanage K, Ishigami-Yuasa M, 
            Okuno Y, Hata S, Nishina H, Hata Y
  TITLE     A cell-based assay to screen stimulators of the Hippo pathway 
            reveals the inhibitory effect of dobutamine on the YAP-dependent 
            gene transcription.
  JOURNAL   J Biochem. 2011 Aug;150(2):199-208. doi: 10.1093/jb/mvr063. Epub 
            2011 May 17.
  PUBMED    21586534
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1093/jb/mvr063";
            journalName: "J Biochem"; date: "2011-08"; volume: "150"; issue: 
            "2"; pages: "199-208"
COMMENT     Sequence Label: pClneoMyc-LATS1
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          150..282
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     265..284
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        327..345
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             363..392
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ccgtcagatc actagaagct ttattgcggt agtttatcac agttaaattg ctaacgcagt
       61 cagtgcttct gacacaacag tctcgaactt aagctgcagt gactctctta aggtagcctt
      121 gcagaagttg gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag
      181 gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac
      241 ctattggtct tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca
      301 attacagctc ttaaggctag agtacttaat acgactcact ataggctaga ccccccaaca
      361 tggagcagaa gcttatcagc gaggaggacc tgctcgagga attcacgcgt atgaagagga
      421 gtgaaaagcc agaaggatat agacaaatga ggcctaagac ctttcctgcc agtaactata
      481 ctgtcagtag ccggcaaatg ttacaagaaa ttcgggaatc ccttaggaat ttatctaaac
      541 tatctgatgc tgctaaggct gagcataaca tgagtaaaat gtcaaccgaa gatcctcgac
      601 aagtcagaaa tccacccaaa tttgggacgc atcataaagc cttgcaggaa attcgaaact
      661 ctctgcttcc atttgcaaat gaaacaaatt cttctcggag tacttcagaa gttaatccac
      721 aaatgcttca agacttgcaa gctgctggat ttgatgagga tatggttat
//
