LOCUS       sequence-118420-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Myc-tagged LATS2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Bao Y, Nakagawa K, Yang Z, Ikeda M, Withanage K, Ishigami-Yuasa M, 
            Okuno Y, Hata S, Nishina H, Hata Y
  TITLE     A cell-based assay to screen stimulators of the Hippo pathway 
            reveals the inhibitory effect of dobutamine on the YAP-dependent 
            gene transcription.
  JOURNAL   J Biochem. 2011 Aug;150(2):199-208. doi: 10.1093/jb/mvr063. Epub 
            2011 May 17.
  PUBMED    21586534
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1093/jb/mvr063";
            journalName: "J Biochem"; date: "2011-08"; volume: "150"; issue: 
            "2"; pages: "199-208"
COMMENT     Sequence Label: pClneoMyc-LATS2
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          151..283
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     266..285
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        328..346
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             364..393
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 accgtcagat cactagaagc tttattgcgg tagtttatca cagttaaatt gctaacgcag
       61 tcagtgcttc tgacacaaca gtctcgaact taagctgcag tgactctctt aaggtagcct
      121 tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa
      181 ggagaccaat agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca
      241 cctattggtc ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc
      301 aattacagct cttaaggcta gagtacttaa tacgactcac tataggctag accccccaac
      361 atggagcaga agcttatcag cgaggaggac ctgctcgagg aattcacgcg tatgaggcca
      421 aagacttttc ctgccacgac ttattctgga aatagccggc agcgactgca agagattcgt
      481 gaggggttaa agcagccatc caagtcttcg gttcaggggc tacccgcagg accaaacagt
      541 gacacttccc tggatgccaa agtcctgggg agcaaagatg ccaccaggca gcagcagcag
      601 atgagagcca ccccaaagtt cggaccttat cagaaagcct tgagggaaat cagatattcc
      661 ttgttgcctt ttgctaatga atcgggcacc tctgcagctg cagaagtgaa ccggcaaatg
      721 ctgcaggaac tggtgaacgc aggatgcgac caggagatgg ctggccgagc tctcaagcag
      781 actggcagca ggagcatcga ggccgccctg gagtacatca g
//
