LOCUS       sequence-118422-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses two dimeric Broccoli aptamers in the F30 scaffold in 
            bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Filonov GS, Jaffrey SR
  TITLE     RNA Imaging with Dimeric Broccoli in Live Bacterial and Mammalian 
            Cells.
  JOURNAL   Curr Protoc Chem Biol. 2016 Mar 16;8(1):1-28. doi: 
            10.1002/9780470559277.ch150174.
  PUBMED    26995352
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/9780470559277"; journalName: "Curr Protoc Chem Biol"; date:
            "2016-03-16- 16"; volume: "8"; issue: "1"; pages: "1-28"
COMMENT     Sequence Label: pET28c-F30-2xdBroccoli
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      214..261
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             268..285
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(338..356)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      352..399
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      436..864
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(523..542)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     733..754
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggtcgggtcc atctgagacg gtcgggtcca gatattcgta tctgtcgagt agagtgtggg
       61 ctcagatgtc gagtagagtg tgggctccca catactctga tgatccagac ggtcgggtcc
      121 atctgagacg gtcgggtcca gatattcgta tctgtcgagt agagtgtggg ctcagatgtc
      181 gagtagagtg tgggctggat cattcatggc aagctagcat aaccccttgg ggcctctaaa
      241 cgggtcttga ggggtttttt gctcgagcac caccaccacc accactgaga tccggctgct
      301 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
      361 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
      421 ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
      481 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
      541 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
      601 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
      661 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
      721 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
      781 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
      841 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttaca
//
