LOCUS       sequence-118423-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Wild-type voltage sensing domain from Ciona voltage sensing 
            phosphatase fused to super ecliptic A227D with restriction site 
            flanking each transmembrane domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Piao HH, Rajakumar D, Kang BE, Kim EH, Baker BJ
  TITLE     Combinatorial mutagenesis of the voltage-sensing domain enables the 
            optical resolution of action potentials firing at 60 Hz by a 
            genetically encoded fluorescent sensor of membrane potential.
  JOURNAL   J Neurosci. 2015 Jan 7;35(1):372-85. doi: 
            10.1523/JNEUROSCI.3008-14.2015.
  PUBMED    25568129
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3008-14"; journalName: "J Neurosci"; date: 
            "2015-01-7- 7"; volume: "35"; issue: "1"; pages: "372-85"
COMMENT     Sequence Label: CC1
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgccacca tggagggatt cgacggttca gattttagtc
      121 ctccagctga tttagttggc gttgacggtg cagtcatgcg gaacgtcgtt gacgtcacga
      181 taaatggtga cgtcactgct ccgccgaaag ctgcgccaag aaaaagtgaa tcggtaaaga
      241 aagttcattg gaatgatgta gaccaaggac cgagtgaaaa accagagaca agacaggagg
      301 aacgaataga tatacccgag atttcaggtc tatggtgggg cgagaatgaa catggagtgg
      361 acgatgggag aatggagata cctactactg gtgtaggtcg cgtccagttt cggatccgag
      421 cagtgattga tcatctaggg atgcgagtct ttggagtctt cctaattttc ttggacatca
      481 tcctcatgat cattgatctc agtcttatcg ataaaagtga atcttcacaa tccttttatg
      541 acgggatggc tttggctctt tcttgttatt tcatgctgga tttaggatta aggatatttg
      601 cctacgggcc caagcttttc ttcaccaacc cttgggaggt ggctgatggt ttgattatcg
      661 ttgtcacatt cgtcgtcacg atattttaca ctgtgttaga tgaatacggt accgaaacag
      721 gagccgatgg
//
