LOCUS       sequence-118424-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Myc-tagged C.elegans YAP-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Iwasa H, Maimaiti S, Kuroyanagi H, Kawano S, Inami K, Timalsina S, 
            Ikeda M, Nakagawa K, Hata Y
  TITLE     Yes-associated protein homolog, YAP-1, is involved in the 
            thermotolerance and aging in the nematode Caenorhabditis elegans.
  JOURNAL   Exp Cell Res. 2013 Apr 15;319(7):931-45. doi: 
            10.1016/j.yexcr.2013.01.020. Epub 2013 Feb 7.
  PUBMED    23396260
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.yexcr.2013.01.020"; journalName: "Exp Cell Res"; date: 
            "2013-04-15- 15"; volume: "319"; issue: "7"; pages: "931-45"
COMMENT     Sequence Label: pClneoMyc'-cYAP-1
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          151..283
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     266..285
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        328..346
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             367..396
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 accgtcagat cactagaagc tttattgcgg tagtttatca cagttaaatt gctaacgcag
       61 tcagtgcttc tgacacaaca gtctcgaact taagctgcag tgactctctt aaggtagcct
      121 tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa
      181 ggagaccaat agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca
      241 cctattggtc ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc
      301 aattacagct cttaaggcta gagtacttaa tacgactcac tataggctag cagacccccc
      361 aacatggagc agaagcttat cagcgaggag gacctgctcg aggaattcac gcgtatggcg
      421 tccaagagca ttcataagaa gcaccaagaa aattcacagc aagataaaaa ccaattctca
      481 gtgcatcatt atttagatcc aaatcagagt attcatgctt taatttcatg ctcagaaaaa
      541 aagtatgaga aaaatcgaaa tcaaaagaaa aatccactac cttcatcata ttaccatcag
      601 aagcggaatc cgggaagcag tgcacattcc ccttatggat cagtagatga aagtagtcga
      661 acagccgtca gcccagctat ggatatggta tcaaaccaag caccgattca cacacgacaa
      721 gtcagtgcac cgaaccttca tacgagtgtt aataatggcc aatcttccgc aacagtacca
      781 catccatctc atcacaatgt ccaccatcaa cactcaaaaa gtgtttctgc attaccaatg
      841 actattggct actctccagt accttctcac gtaa
//
