LOCUS       sequence-118426-         664 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent viral expression of ChR2(H134R)-mCherry for neuronal 
            excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Atasoy D, Aponte Y, Su HH, Sternson SM
  TITLE     A FLEX switch targets Channelrhodopsin-2 to multiple cell types for 
            imaging and long-range circuit mapping.
  JOURNAL   J Neurosci. 2008 Jul 9. 28(28):7025-30.
  PUBMED    18614669
REFERENCE   2  (bases 1 to 664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2008 Jul 9. 28(28):7025-30."
COMMENT     Sequence Label: AAV-FLEX-rev-ChR2(H134R)-mCherry
FEATURES             Location/Qualifiers
     source          1..664
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..105
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      123..132
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgggtcgatg gtgagatctg gactagaggg tcgatggtga tgcttggatc aaaataggaa
       61 gaatcccaat gataacttcg tataaagtat cctatacgaa gttatactag aattcggtac
      121 cggccaccat ggactatggc ggcgctttgt ctgccgtcgg acgcgaactt ttgttcgtta
      181 ctaatcctgt ggtggtgaac gggtccgtcc tggtccctga ggatcaatgt tactgtgccg
      241 gatggattga atctcgcggc acgaacggcg ctcagaccgc gtcaaatgtc ctgcagtggc
      301 ttgcagcagg attcagcatt ttgctgctga tgttctatgc ctaccaaacc tggaaatcta
      361 catgcggctg ggaggagatc tatgtgtgcg ccattgaaat ggttaaggtg attctcgagt
      421 tcttttttga gtttaagaat ccctctatgc tctaccttgc cacaggacac cgggtgcagt
      481 ggctgcgcta tgcagagtgg ctgctcactt gtcctgtcat ccttatccgc ctgagcaacc
      541 tcaccggcct gagcaacgac tacagcagga gaaccatggg actccttgtc tcagacatcg
      601 ggactatcgt gtggggggct accagcgcca tggcaaccgg ctatgttaaa gtcatcttct
      661 tttg
//
