LOCUS       sequence-118427-         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express FLAG-tagged YAP1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Bao Y, Nakagawa K, Yang Z, Ikeda M, Withanage K, Ishigami-Yuasa M, 
            Okuno Y, Hata S, Nishina H, Hata Y
  TITLE     A cell-based assay to screen stimulators of the Hippo pathway 
            reveals the inhibitory effect of dobutamine on the YAP-dependent 
            gene transcription.
  JOURNAL   J Biochem. 2011 Aug;150(2):199-208. doi: 10.1093/jb/mvr063. Epub 
            2011 May 17.
  PUBMED    21586534
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1093/jb/mvr063";
            journalName: "J Biochem"; date: "2011-08"; volume: "150"; issue: 
            "2"; pages: "199-208"
COMMENT     Sequence Label: pFLAG-YAP1
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..47
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accgtcagaa ttgatctacc atggactaca aagacgatga cgacaagctt gcggccgcga
       61 attcatcgat agatcccggg cagcagccgc cgcctcaacc ggccccccag ggccaagggc
      121 agccgccttc gcagcccccg caggggcagg gcccgccgtc cggacccggg caaccggcac
      181 ccgcggcgac ccaggcggcg ccgcaggcac cccccgccgg gcatcagatc gtgcacgtcc
      241 gcggggactc ggagaccgac ctggaggcgc tcttcaacgc cgtcatgaac cccaagacgg
      301 ccaacgtgcc ccagaccgtg cccatgaggc tccggaagct gcccgactcc ttcttcaagc
      361 cgccggagcc caaatcccac tcccgacagg ccagtactga tgcaggcact gcaggagccc
      421 tgactccaca gcatgttcga gctcattcct ctccagcttc tctgcagttg ggagctgttt
      481 ctcctgggac actgaccccc actggagtag tctctggccc agcagctaca cccacagctc
      541 agcatcttcg acagtcttct tttgagatac ctgatgatgt acctctgcca gcaggttggg
      601 agatggcaaa gacatcttct ggtcagagat acttcttaaa tcacatcgat cagacaacaa
      661 catggcagga ccccaggaag gccatgctgt cccagatgaa cgtcacagcc cccaccagtc
      721 ca
//
