LOCUS       sequence-118429-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Voltage sensing domain of Ciona with the D136A/A154D/E183N/R223Q 
            mutations fused to super ecliptic pHlorin A227D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Piao HH, Rajakumar D, Kang BE, Kim EH, Baker BJ
  TITLE     Combinatorial mutagenesis of the voltage-sensing domain enables the 
            optical resolution of action potentials firing at 60 Hz by a 
            genetically encoded fluorescent sensor of membrane potential.
  JOURNAL   J Neurosci. 2015 Jan 7;35(1):372-85. doi: 
            10.1523/JNEUROSCI.3008-14.2015.
  PUBMED    25568129
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3008-14"; journalName: "J Neurosci"; date: 
            "2015-01-7- 7"; volume: "35"; issue: "1"; pages: "372-85"
COMMENT     Sequence Label: CC2
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..63
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(147..167)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     762..790
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
       61 aggctgatca gcgggtttaa acgggccctc tagactcgag tcatcatttg tatagttcat
      121 ccatgccatg tgtaatccca tcagctgtta caaactcaag aaggaccatg tggtctctct
      181 tttcgttggg atctttcgaa agagtagaag ttgtaaacag gtaatggttg tctggtaaaa
      241 ggacgggccc atcgccaatt ggagtatttt gttgataatg gtctgctagt tgaacgcctc
      301 catcttcaat gttgtgtcta attttgaagt tagctttgat tccattcttt tgtttgtctg
      361 ccatgatgta cacctggtga tcgttatagt tgtattccaa tttgtgtcca agaatgtttc
      421 catcttcttt aaaatcaata ccttttaact cgattctatt aacaagggta tcaccttcaa
      481 acttgacttc agcacgtgtc ttgtagttcc cgtcatcttt gaaaaatata gttctttcct
      541 gtacataacc ttcgggcatg gcactcttga aaaagtcatg ccgtttcata tgatctgggt
      601 atcttgaaaa gcattgaaca ccataagtta aagtagtgac aagtgttggc catggaacag
      661 gtagttttcc agtagtgcaa ataaatttaa gggtaagttt tccgtatgtt gcatcacctt
      721 caccctctcc actgacagaa aatttgtgcc cattaacatc accatctaat tcaacaagaa
      781 ttgggacaac tccagtg
//
