LOCUS       sequence-118430-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CAS9 in Leishmania.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Zhang WW, Matlashewski G
  TITLE     CRISPR-Cas9-Mediated Genome Editing in Leishmania donovani.
  JOURNAL   MBio. 2015 Jul 21;6(4). pii: e00861-15. doi: 10.1128/mBio.00861-15.
  PUBMED    26199327
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2015-07-21"; volume: "6(4). pii: e00861-15. doi"; pages: " 
            10.1128/mBio.00861-15"
COMMENT     Sequence Label: pLPhygCAS9
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             273..320
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(348..365)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 agagagtgat cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc
       61 gggataagcc catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc
      121 tgggagcccc tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca
      181 gcaccaaaga ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga
      241 cacggatcga cctgtctcag ctgggaggcg acaaaaggcc ggcggccacg aaaaaggccg
      301 gccaggcaaa aaagaaaaag taagaattcc tagagctcgc tgatcagcct cgactgtgcc
      361 ttctagttgc cagccatctg ttggatccgc ggccccgcct cgagcccggg cgccgtcgac
      421 cgctgcttgc gttcgcatgg acgccacgtt cgacccgatc gggcagcagg ccgcggagac
      481 gcggcagcgc aaacgtgccc ggcgggtggc tcgttccgga ggaggcgttc cgccgtcgct
      541 acgtcagcat gcaagaggat cattgctttg cggttgaggt ggcggagctc aactaccagc
      601 gtgccgtgga atacgttcat caagcgtcag ccgccggcgc ggtcgcgaca cctgctcggc
      661 ggagcggcca ctgtgctgct ctcttgacgg ccggtcgcga cgcagggccg ctggtgggtc
      721 tccagcgggt acggtgcgat caggcagcca tgacggctac gctgagcacg cttgcgaagg
      781 agctggctgc actgtcgccg tagcgcgatg cgagacgtat cgagcgcatg ccagtggg
//
