LOCUS       sequence-118434-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNA in Leishmania.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Zhang WW, Matlashewski G
  TITLE     CRISPR-Cas9-Mediated Genome Editing in Leishmania donovani.
  JOURNAL   MBio. 2015 Jul 21;6(4). pii: e00861-15. doi: 10.1128/mBio.00861-15.
  PUBMED    26199327
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2015-07-21"; volume: "6(4). pii: e00861-15. doi"; pages: " 
            10.1128/mBio.00861-15"
COMMENT     Sequence Label: pSPneogRNAH
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(127..194)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     misc_RNA        complement(201..276)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(493..511)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(494..511)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(592..611)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     711..733
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 atcatgcgaa ttcgagctct ggagagagga gagaggagag agaggggaag gagaagagag
       61 agctctggag agaggagaga ggagagagag gggaaggaga agagagagct cggtacccgg
      121 ggatccgtcc cattcgccat gccgaagcat gttgcccagc cggcgccagc gaggaggctg
      181 ggaccatgcc ggccaaaaaa gcaccgactc ggtgccactt tttcaagttg ataacggact
      241 agccttattt taacttgcta tttctagctc taaaacaggt cttctcgaag acccacaaaa
      301 tcaccaaacc aaaaactcaa ccgagccccc gataagatcc acacatcacg aacacacctc
      361 aaattctcac aagcccaccc tctcactact tccacctaaa aaagctctac ccaccccgtt
      421 cataccccct cccccttgca actcctacgt caatcgcaga cctcataact cacaagcttc
      481 agctgctcga gttctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta
      541 ttaattgtag ccgcgttcta acgacaatat gtccatatgg tgcactctca gtacaatctg
      601 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
      661 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      721 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaa
//
