LOCUS       sequence-118435-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl4 with C-term V5 and His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST40-ARL4-V5-His6
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..19)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(29..70)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    96..120
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(793..812)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(794..812)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(857..901)
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
ORIGIN
        1 catggtgatg gtgatgatga ccggtacgcg tagaatcgag accgaggaga gggttaggga
       61 taggcttacc ttcgaaccgc gggccctcta gatcaaccac tttgtacaag aaagctgggt
      121 ctcttttctt tttctgttgc cgcaacattt ttcttctttt aatgatcata tcatgtagtt
      181 tctcaagtcc ttcctttagg ccatctccta tgattgcaca ggtaggctgc aaatgccaag
      241 gagttgatga gctcagttca cccattgcta acaatttctc aatttctgaa agtgacaatg
      301 agttcctcaa atcttgtttg ttagcaacta taagtacagg gactccctga ttttctgata
      361 tcctagttat tttgtgaagt tcagttttgg cttcttccat cctttcgaca tcaacagagt
      421 ccacaacaaa tacaatgcca tctgtgcatc tggtatatga cttccacagt ggccttaatt
      481 tctcctgacc acctacatcc cagaagtgaa aagtgactgt tttagaattt cccaaggtta
      541 ccttaatttt ctcagtgtta aatcctttgg taggtacggt atttacaaat tcattgaact
      601 gcagcctgta taagacagtt gtctttccag cacagtccaa acccagaata acaatgtgga
      661 aagactgaaa tgaaggcagg ttggacagga tagaagtctg gtctgacagc ccattcccca
      721 tggttctatc tccttctaag cctgcttttt tgtacaaaac ttgttgatag cttaactagc
      781 cagcttgggt ctccctatag tgagtcgtat taatttcgat aagccagtaa gcagtgggtt
      841 ctctagttag ccagagagct ctgcttatat agacctccca ccgtacacgc ctacggccca
      901 t
//
