LOCUS       sequence-118438-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bleomycin drug selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Zhang WW, Matlashewski G
  TITLE     CRISPR-Cas9-Mediated Genome Editing in Leishmania donovani.
  JOURNAL   MBio. 2015 Jul 21;6(4). pii: e00861-15. doi: 10.1128/mBio.00861-15.
  PUBMED    26199327
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2015-07-21"; volume: "6(4). pii: e00861-15. doi"; pages: " 
            10.1128/mBio.00861-15"
COMMENT     Sequence Label: pSPble
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..407)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(706..724)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(707..724)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(805..824)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgtcggac cggcggtgtt ggtcggcgtc ggtcagtcct gctcctcggc cacgaagtgc
       61 acgcagttgc cggccgggtc gcgcagggcg aactcccgcc cccacggctg ctcgccgatc
      121 tcggtcatgg ccggcccgga ggcgtcccgg aagttcgtgg acacgacctc cgaccactcg
      181 gcgtacagct cgtccaggcc gcgcacccac acccaggcca gggtgttgtc cggcaccacc
      241 tggtcctgga ccgcgctgat gaacagggtc acgtcgtccc ggaccacacc ggcgaagtcg
      301 tcctccacga agtcccggga gaacccgagc cggtcggtcc agaactcgac cgctccggcg
      361 acgtcgcgcg cggtgagcac cggaacggca ctggtcaact tggccatggt gatatctagt
      421 tggggatctg atatcatcga tgaattcgag ctctggagag aggagagagg agagagaggg
      481 gaaggagaag agagagctct ggagagagga gagaggagag agaggggaag gagaagagag
      541 agctcggtac ccgatccgat gaagatccgc agcgtgcgtc cgcttgtggt gttgctggtg
      601 tgcgtcgcgg cggtgctcgc actcagcgcc tccgctgagc cgcacaaggc ggccggggat
      661 cctctagagt cgacctgcag gcatgcaagc ttcagctgct cgagttctat agtgtcacct
      721 aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa
      781 tatgtccata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
      841 c
//
