LOCUS       sequence-118439-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArf4 with C-term V5 and His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST40-ARF4-V5-His6
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(28..69)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(678..702)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(731..750)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(732..750)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(795..846)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(824..844)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atggtgatgg tgatgatgac cggtacgcgt agaatcgaga ccgaggagag ggttagggat
       61 aggcttacct tcgaaccgcg ggccctctag atcaaccact ttgtacaaga aagctgggtc
      121 acgttttgaa agctcatttg acagccagtc aagtccttca tacagaccag ttccttgtgt
      181 tgcacaagtg gcttgaacat accatgttct gttacgaaga gactgaagcc ctagtttatc
      241 tgtcatttca ctgatggcca tagcatttgg caaatcctgt ttgtttgcaa aaagtagcag
      301 cactgcatct ctcaattcat ctaccagaag cattttctgc agctcatctg ctacttcctg
      361 aattctttca cgatcgttgc tatctaccac aaaaataaga ccctgggtat tctggaagta
      421 atgcttccag agaggcctaa ttctatcttg accaccaaca tcccatactg tgaaacaaat
      481 gttcttatat tctactgttt ccacattaaa accaatggta ggaatggtgg tgactatctc
      541 ccctaacttc agtttataca gaatggttgt cttgccagca gcatccaatc caaccatcaa
      601 aatgcgcatc tgcttcttgc caaatagtcg ggagaagagg gaggagatag tgaggcccat
      661 ggttctatct ccttctaagc ctgctttttt gtacaaactt gttgatagct taactagcca
      721 gcttgggtct ccctatagtg agtcgtatta atttcgataa gccagtaagc agtgggttct
      781 ctagttagcc agagagctct gcttatatag acctcccacc gtacacgcct accgcccatt
      841 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt ccc
//
