LOCUS       sequence-118440-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArf1 with C-term V5 and His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST40-ARF1-V5-His6
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(28..69)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(681..705)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(734..753)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(735..753)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atggtgatgg tgatgatgac cggtacgcgt agaatcgaga ccgaggagag ggttagggat
       61 aggcttacct tcgaaccgcg ggccctctag atcaaccact ttgtacaaga aagctgggtc
      121 cttctggttc cggagctgat tggacagcca gtccagtcct tcatagagcc cgtcgccgct
      181 ggtggcgcag gtggcctgaa tgtaccagtt cctgtggcgt agtgagtgca gccccagctt
      241 gtctgtgatc tcggccgcat tcatggcgtt ggggaggtcc tgcttgttgg cgaacaccag
      301 gaggacagca tcccggagct cgtcctcggc cagcatcctc atgagctcct cacgggcctc
      361 gttcacacgc tctctgtcat tgctgtccac cacgaagatc aggccttgtg tgttctggaa
      421 gtagtggcgc cacaggggcc ggatcttgtc ctggccaccc acgtcccaca cagtgaagct
      481 gatgttcttg tactccacgg tttccacgtt gaagcctatg gtgggaatgg tggtcacgat
      541 ctcacccagc ttaagcttgt agaggatcgt ggtcttccct gcagcatcca ggcccaccat
      601 gaggatgcgc atttcttttt tgccaaaaag gcccttgaag aggttggcga agatgttccc
      661 catggttcta tctccttcta agcctgcttt tttgtacaaa cttgttgata gcttaactag
      721 ccagcttggg tctccctata gtgagtcgta ttaatttcga taagccagta agca
//
