LOCUS       sequence-118442-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual expression of GBD-LUMP and cdc42.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Hoepker AC, Wang A, Le Marois A, Suhling K, Yan Y, Marriott G
  TITLE     Genetically encoded sensors of protein hydrodynamics and molecular 
            proximity.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 30. pii: 201424021.
  PUBMED    25931526
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Apr 30. pii: 201424021."
COMMENT     Sequence Label: 213-petDuet1-GBD-LUMP--cdc42
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..50)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(72..647)
                     /codon_start=1
                     /product="human Rho family small GTPase Cdc42"
                     /label=Cdc42
                     /translation="MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVM
                     IGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHH
                     CPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQK
                     GLKNVFDEAILAALEPPEPKKSRRCVLL"
     protein_bind    690..714
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(714..733)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(715..733)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt accagactcg
       61 agtgcggccg ctcatagcag cacacacctg cggctcttct tcggttctgg aggctccagg
      121 gcagccaata ttgcttcgtc aaatacattc tttaggcctt tctgtgtaag tgcagaacac
      181 tccacatact tgacagcctt caggtcacgg gccagctttt cagcagtctc tggagtgata
      241 ggcttctgtt tgttcttggc aagtttctca atagtagagg ggtcatctct gagatcaatt
      301 tgagtcccaa caagcaagaa aggagtcttt ggacagtggt gagttatctc aggcacccac
      361 ttttctttca cgttttcaaa tgaagatgga gagaccactg aaaaacagac tagaaataca
      421 tctgtttgtg gataactcag cggtcgtaat ctatcataat cctcttgccc tgcagtatca
      481 aaaagtccaa gagtatatgg ttctccacca atcataactg tgactgcata gttgtcaaaa
      541 acagtcggta catattccga tggaaatttg tttgttgtgt aggatatcag gagacatgtt
      601 ttaccaacag caccatcgcc cacaacaaca cacttaattg tctgcatatg tatatctcct
      661 tcttatactt aactaatata ctaagatggg gaattgttat ccgctcacaa ttcccctata
      721 gtgagtcgta ttaatttcga ttatgcggcc gtgtacaata cgattacttt ctgttcgact
      781 taagcattat gcggccgcaa gcttgagtgc ggccgcctac cattcatt
//
