LOCUS       sequence-118444-         611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid for bacterial overexpression of RBPMS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pet23a_RBPMS_20_101
FEATURES             Location/Qualifiers
     source          1..611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..35
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             327..344
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(397..415)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      411..458
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(582..601)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atatggctag catgactggt ggacagcaaa tgggtcgcgg atccgaattc gagctccgtc
       61 gacccatgga ggaggaggtc cggaccctat ttgtcagtgg ccttcctctg gatatcaaac
      121 ctcgggagct ctatctgctt ttcagaccat ttaagggcta tgagggttct cttataaagc
      181 tcacatctaa acagcctgta ggttttgtca gttttgacag tcgctcagaa gcagaggctg
      241 caaagaatgc tttgaatggc atccgcttcg atcctgaaat tccgcaaaca ctacgactag
      301 agtttgctaa ggcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta
      361 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      421 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      481 gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
      541 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
      601 ttcctttctc g
//
