LOCUS       sequence-118446-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TetActivator protein under the control of the c-fos 
            promoter for use in TetTaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Zhang Z, Ferretti V, Guntan I, Moro A, Steinberg EA, Ye Z, Zecharia 
            AY, Yu X, Vyssotski AL, Brickley SG, Yustos R, Pillidge ZE, Harding 
            EC, Wisden W, Franks NP
  TITLE     Neuronal ensembles sufficient for recovery sleep and the sedative 
            actions of alpha2 adrenergic agonists.
  JOURNAL   Nat Neurosci. 2015 Apr;18(4):553-61. doi: 10.1038/nn.3957. Epub 2015
            Feb 23.
  PUBMED    25706476
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3957"; 
            journalName: "Nat Neurosci"; date: "2015-04"; volume: "18"; issue: 
            "4"; pages: "553-61"
COMMENT     Sequence Label: pAAV-cFos-tTA-pA
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        94..112
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     94..111
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(182..208)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(342..365)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(366..386)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttatatttac cttagagctt taaatctctg taggtagttt gtccaattat gtcacaccac
       61 agaagtaagg ttccttcaca aagatcctct agcatttagg tgacactata gaatagggcc
      121 ctctagatgc atgctcgagc ggccggccgc cagtgtgatg gatggataat tcttagtcga
      181 gtgcgtagtc tggtacgtcg tacggatagt cgagatcctt atcacagcat gtccaggtcg
      241 aagtcgtcca gggcgtcggc cggcagcatg tccaggtcga agtcgtccag ggcgtcggcc
      301 ggcagcatgt ccaggtcgaa gtcgtccagg gcgtcggccg gcttgtcgtc gtcgtccttg
      361 tagtcgacct tgcgcttctt cttgggggag ccggactcgc acttcagctg cttctccagg
      421 ccgcagatga tcagctccag gccgaacagg aaggcgggct cggcgccctg gtggtcgaac
      481 agctcgatgg cctggcgcag cagggggggc atggagtcgg tggtgggggt ctcgcgctcc
      541 tccttggcca cctggtgctc ctggtcctcc agcacgcagc ccagggtgaa gtggcccacg
      601 gcggacaggg cgtacagggc gttctccagg gagaagccct gctggcacag gaaggccagc
      661 tggttctcca gggtctcgta ctgcttctcg gtggggcggg tgcccaggtg caccttggcg
      721 ccgtcgcggt gggacagcag tgcgcagcgg aaggacttgg cgttgttgcg caggaagtcc
      781 tgccaggact cgccctccag ggggcagaag tgggtgtggt ggcggtccag catctcgatg
      841 gccagggcgt ccagcagg
//
