LOCUS       sequence-118448-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express GFP-fused TAZ by lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Bao Y, Nakagawa K, Yang Z, Ikeda M, Withanage K, Ishigami-Yuasa M, 
            Okuno Y, Hata S, Nishina H, Hata Y
  TITLE     A cell-based assay to screen stimulators of the Hippo pathway 
            reveals the inhibitory effect of dobutamine on the YAP-dependent 
            gene transcription.
  JOURNAL   J Biochem. 2011 Aug;150(2):199-208. doi: 10.1093/jb/mvr063. Epub 
            2011 May 17.
  PUBMED    21586534
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1093/jb/mvr063";
            journalName: "J Biochem"; date: "2011-08"; volume: "150"; issue: 
            "2"; pages: "199-208"
COMMENT     Sequence Label: pLL3.7 EGFPC2 TAZ
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    79..112
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    140..261
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(177..196)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     231..250
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattgtac ctagtggaac
       61 cggaaccctt aaacatgtat aacttcgtat aatgtatgct atacgaagtt attaggtccc
      121 tcgacgaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
      181 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
      241 caaactcatc aatgtatctt atcatgtctg ctcgaagcat taaccctcac taaagggaag
      301 cggccgcccg ggtcgagttt tttttttttt ttttttggat tcaaaattac attgtattcc
      361 atacagtaga attttactat ccatacaatg atttttaaag ctcaagttaa atagttttta
      421 aagcatttgg tactactgtc atcaatacag tttttgaaac tgtaaatcag gtcgaatttt
      481 gtgcacattt cctggaccaa gatgccctca gaagtaactg cctgtggtca gctttttatg
      541 gtttaaaatc aattggtgta taaatttcaa ttaacaccca taaagcttag ccatggggca
      601 gcagagaaga aagagaaaag tattctgcat aatcaatcct gcagacacaa ttctgtataa
      661 tctgtcacaa gaacgcaggc ttgcagaaaa tgaaaataga atatttattt atgtttaact
      721 taagttactc tcaatcaaaa ccaggcaatg attaaactgg caacataaaa aggagggagc
      781 acgagtcatg gaggcggga
//
