LOCUS       sequence-118452-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl4c with C-term V5 and His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST40-ARL4C-V5-His6
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(28..69)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(714..738)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(767..786)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(768..786)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(831..875)
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
ORIGIN
        1 atggtgatgg tgatgatgac cggtacgcgt agaatcgaga ccgaggagag ggttagggat
       61 aggcttacct tcgaaccgcg ggccctctag atcaaccact ttgtacaaga aagctgggtc
      121 ccgcttcttc ttctgcttga gggacttcct gcgtttcagg atcatctcat agagcttgtc
      181 catgccctcg gtgaggccct cgccgatgat ggcgcacgcc ggctggacgt gataggtggt
      241 ggccgggata agctcgtgca gcgccagctg cttctcaatc tctgccaccg gcagcgactt
      301 gggcaggtcc tgcttgttgg cgatgaccag cagcggcgtg ccctggttct cggcgaactt
      361 ggtcaccttg tgcagctccg tcttggcctc ctccagccgg tccacgtcca ccgagtccac
      421 cacgtagatg atgccgtccg tgcagcggct gtaggacttc cacagcggcc gcagcttctc
      481 ctggccgccc acgtcccaga agtggcagct gatgcccttg gccgtgccgt tgctcagctt
      541 gatcttctcg gtgttgaagc cgatggtggg caccgtgttc acgaactcgt tgaacttgag
      601 ccggtagagc accgtggtct tgccggccga gtccaagccc aacatgacga tatgcaggga
      661 ctggaaggcc gagatgttag aggagatgtt gcccatggtt ctatctcctt ctaagcctgc
      721 ttttttgtac aaacttgttg atagcttaac tagccagctt gggtctccct atagtgagtc
      781 gtattaattt cgataagcca gtaagcagtg ggttctctag ttagccagag agctctgctt
      841 atatagacct tcccaccgta cacgcctacc gcccaatttg cgtcaa
//
