LOCUS       sequence-118456-         538 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl5b with C-term V5 and His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST40-ARL5B-V5-His6
FEATURES             Location/Qualifiers
     source          1..538
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..20)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(30..71)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    97..121
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcatggtgat ggtgatgatg accggtacgc gtagaatcga gaccgaggag agggttaggg
       61 ataggcttac cttcgaaccg cgggccctct agatcaacca ctttgtacaa gaaagctggg
      121 tctctcacac caatccggga ggtcatccac tctagacctt ggcataaccc ttctcctgtg
      181 agagcacagc aggattgaat gtgccatgga tgatccttaa ttgaactaag ggtgaggtat
      241 ttcgagattt cagctgctgt catacaccct ttcatatcct gtttatttgc aaagataagg
      301 actgcagcct tccgtaaatc ctcatgagcc aacattctgt ataattcttc ttttgtaata
      361 gctagtcgtt ccctgtcaat gctatcaaca acaagaatga tgaactctgt atttgagtaa
      421 tatgtgttcc aggatgatcg cagagactcc tgaccaccaa tatcccacat aagaaaatga
      481 gtgttcttca caactatttc ttcaacattg cttcctatgg ttggagaagt atgaacca
//
