LOCUS       sequence-118459-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl11 with C-term V5 and His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST40-ARL11-V5-His6
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(28..69)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(726..750)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(779..798)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(780..798)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(843..894)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(872..892)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atggtgatgg tgatgatgac cggtacgcgt agaatcgaga ccgaggagag ggttagggat
       61 aggcttacct tcgaaccgcg ggccctctag atcaaccact ttgtacaaga aagctgggtc
      121 agatctcttg ctgtctccgc gctcagcccc atgggctctc gcctgcagac acatgcagct
      181 gcgagatttc aggaggctcc acaggctctg cagggcctcg ggcagcccct ccccagtgag
      241 ggcactgcag ccccggagct cccagcggtg gtcctggaat ctctctagac tcagcctgtt
      301 tctgatctta agcagcggaa gtgcatcagg tgcctcctgc ttgttggcca gcaccaagaa
      361 ggggacgcca gccatgttgg ggtcgttcag gacttctgtg agctcagccg ccgactcggg
      421 taagcgggct tcatctgtgc tgtccagcac gtacacgagg atatctgtgc cttccagata
      481 gtccttccag ctggctctga gcggggcctg ccccccaacg tcccagagag tcagtgacac
      541 gtgcccagga gctttcagag gctccacgtt gaaaccaaca gtgggcaggg tctccaccag
      601 ctggtggccc ttcagcttgt aaaggagcgt ggtcttgccc gccgagtcca ggcccatcat
      661 caccacctgg gcttccgcct tgtgacctct ggaattcaca gaacccatgg ttctatctcc
      721 ttctaagcct gcttttttgt acaaacttgt tgatagctta actagccagc ttgggtctcc
      781 ctatagtgag tcgtattaat ttcgataagc cagtaagcag tgggttctct agttagccag
      841 agagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg
//
