LOCUS       sequence-118466-        5198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible TraJ, TraM expression with arabinose..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Leonard J
  TITLE     Leonard Lab Conjugation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJaM
FEATURES             Location/Qualifiers
     source          1..5198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             688..711
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      1307..1338
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     rep_origin      1849..2393
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2294..2313
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3102..3761
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3168..3187)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      complement(3891..3920)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     misc_feature    3967..3988
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             complement(3989..4867)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4894..5178
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     5101..5120
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 atgtatccga tggatcgtat tcaacaaaaa catgctcgtc aaatagatct gctggaaaat
       61 ctgacggcag ttattcagga ttatccaaat ccagcctgta tcagggacga aactggaaaa
      121 tttatttttt gcaatacgct gtttcatgag tcatttctta cacaagatca aagtgctgaa
      181 aaatggcttc tgtcgcagag agatttttgt gaattgatct ctgtcacaga gatggaagca
      241 tataggaatg agcatacgca tcttaatctt gtagaggatg tttttattca gaatagattc
      301 tggacaatat ctgtccagtc atttcttaat ggacacagaa atattattct gtggcaattt
      361 tatgatgctg ctcatgttcg tcataaagac agttataatc aaaaaacgat tgtcagtgat
      421 gatatcagaa atataatcag aagaatgagt gatgattctt ctgtatcatc atatgtaaat
      481 gatgtctttt acttatatag caccggaatc agtcataatg ctatagcaag aatattaaat
      541 atatccatct ccacatcaaa gaaacacgca tctctgatat gcgactactt ctctgtttct
      601 aataaagatg agttaattat cttactctac aataaaaagt ttatttatta tttatacgag
      661 aaggctatgt gtatcataaa tacgcgtgat tacaaggatg acgacgataa gtaagagctc
      721 aaaaaaggta atctctaatg gctaaggtga acctgtatat cagcaatgat gcctatgaaa
      781 aaataaatgc gattattgag aagcgtcgac aggaaggggc aagggaaaaa gatgtcagtt
      841 tttcagcaac agcttcaatg cttcttgaac tggggcttcg tgtacatgag gctcagatgg
      901 agcgtaaaga gtctgcattt aatcagactg agtttaataa attgcttctt gaatgcgttg
      961 taaaaacaca atcatcagta gcgaaaattt tgggtattga gtctctcagt cctcatgtct
     1021 ccggaaattc aaagtttgaa tatgccaata tggttgaaga tatcagggag aaggtatcat
     1081 ctgagatgga acgatttttt ccaaaaaatg atgatgaata aaagcttcaa ggtgtcacca
     1141 ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc
     1201 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1261 gtaatctgca ggagtcacta agggttagtt agttagatta gcagaaagtc aaaagcctcc
     1321 gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca ctcaaaggcg
     1381 gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta gagatggaat
     1441 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1501 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1561 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1621 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1681 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1741 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1801 tgagttttcg ttccactgag cgtcagaccc cttaataaga tgatcttctt gagatcgttt
     1861 tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg gcggtttttc
     1921 gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag gagcgcagtc
     1981 accaaaactt gtcctttcag tttagcctta accggcgcat gacttcaaga ctaactcctc
     2041 taaatcaatt accagtggct gctgccagtg gtgcttttgc atgtctttcc gggttggact
     2101 caagacgata gttaccggat aaggcgcagc ggtcggactg aacggggggt tcgtgcatac
     2161 agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagacaaa
     2221 cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg
     2281 agggagccgc caggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact
     2341 gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct
     2401 ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca ggaaatctcc
     2461 gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt
     2521 gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg gtgcagcctt
     2581 ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac atagtaagcc
     2641 agtatacact ccgctagcgc tgaggtctgc ctcgtgaaga aggtgttgct gactcatacc
     2701 aggcctgaat cgccccatca tccagccaga aagtgaggga gccacggttg atgagagctt
     2761 tgttgtaggt ggaccagttg gtgattttga acttttgctt tgccacggaa cggtctgcgt
     2821 tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt attcaacaaa
     2881 gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     2941 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgtttac tagaggttga
     3001 tcgggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat
     3061 tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcacgg
     3121 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     3181 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     3241 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     3301 tgaacgctca cccggagttt cgtatggcca tgaaagacgg tgagctggtg atctgggata
     3361 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttcg tccctctgga
     3421 gtgaatacca cgacgatttc cggcagtttc tccacatata ttcgcaagat gtggcgtgtt
     3481 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt tttgtctcag
     3541 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     3601 tcgcccccgt tttcacgatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     3661 tggcgatcca ggttcatcat gccgtttgtg atggcttcca tgtcggccgc atgcttaatg
     3721 aattacaaca gtactgtgat gagtggcagg gcggggcgta ataatactag ctccggcaaa
     3781 aaaacgggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc
     3841 gtttgccacc tgacgtctaa gaaaaggaat attcagcaat ttgcccgtgc cgaagaaagg
     3901 cccacccgtg aaggtgagcc agtgagttga ttgctacgta attagttagt tagcccttag
     3961 tgactcgaat tcgcggccgc ttctagagtt atgacaactt gacggctaca tcattcactt
     4021 tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc
     4081 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc
     4141 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga
     4201 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat
     4261 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac
     4321 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc
     4381 gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc
     4441 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat
     4501 ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt
     4561 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac
     4621 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt
     4681 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt
     4741 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta
     4801 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt
     4861 cagccatact tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca
     4921 gacattgccg tcactgcgtc ttttactggc tcttctcgct aaccaaaccg gtaaccccgc
     4981 ttattaaaag cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa
     5041 gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct
     5101 atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac
     5161 tctctactgt ttctccatac ccgttttttt gggctagc
//
