LOCUS       sequence-118469-        4800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Indicuble TraJ expression with arabinose..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Leonard J
  TITLE     Leonard Lab Conjugation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJ
FEATURES             Location/Qualifiers
     source          1..4800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             688..711
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      909..940
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     rep_origin      1451..1995
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     1896..1915
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             2704..3363
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2770..2789)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      complement(3493..3522)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     misc_feature    3569..3590
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             complement(3591..4469)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4496..4780
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4703..4722
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 atgtatccga tggatcgtat tcaacaaaaa catgctcgtc aaatagatct gctggaaaat
       61 ctgacggcag ttattcagga ttatccaaat ccagcctgta tcagggacga aactggaaaa
      121 tttatttttt gcaatacgct gtttcatgag tcatttctta cacaagatca aagtgctgaa
      181 aaatggcttc tgtcgcagag agatttttgt gaattgatct ctgtcacaga gatggaagca
      241 tataggaatg agcatacgca tcttaatctt gtagaggatg tttttattca gaatagattc
      301 tggacaatat ctgtccagtc atttcttaat ggacacagaa atattattct gtggcaattt
      361 tatgatgctg ctcatgttcg tcataaagac agttataatc aaaaaacgat tgtcagtgat
      421 gatatcagaa atataatcag aagaatgagt gatgattctt ctgtatcatc atatgtaaat
      481 gatgtctttt acttatatag caccggaatc agtcataatg ctatagcaag aatattaaat
      541 atatccatct ccacatcaaa gaaacacgca tctctgatat gcgactactt ctctgtttct
      601 aataaagatg agttaattat cttactctac aataaaaagt ttatttatta tttatacgag
      661 aaggctatgt gtatcataaa tacgcgtgat tacaaggatg acgacgataa gtaaactagt
      721 taaaacgggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg
      781 cgttatgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      841 ggtatcagct cactcaaagg cggtaatctg caggagtcac taagggttag ttagttagat
      901 tagcagaaag tcaaaagcct ccgaccggag gcttttgact aaaacttccc ttggggttat
      961 cattggggct cactcaaagg cggtaatcag ataaaaaaaa tccttagctt tcgctaagga
     1021 tgatttctgc tagagatgga atagactgga tggaggcgga taaagttgca ggaccacttc
     1081 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     1141 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     1201 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     1261 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1321 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1381 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccttaataa
     1441 gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa
     1501 ccgccttgca gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta
     1561 actggcttgg aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc
     1621 atgacttcaa gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt
     1681 gcatgtcttt ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac
     1741 tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt
     1801 gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa
     1861 ggcaggaaca ggagagcgca cgagggagcc gccaggggaa acgcctggta tctttatagt
     1921 cctgtcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg
     1981 cggagcctat ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta agtatcttcc
     2041 tggcatcttc caggaaatct ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa
     2101 cgaccgagcg tagcgagtca gtgagcgagg aagcggaata tatcctgtat cacatattct
     2161 gctgacgcac cggtgcagcc ttttttctcc tgccacatga agcacttcac tgacaccctc
     2221 atcagtgcca acatagtaag ccagtataca ctccgctagc gctgaggtct gcctcgtgaa
     2281 gaaggtgttg ctgactcata ccaggcctga atcgccccat catccagcca gaaagtgagg
     2341 gagccacggt tgatgagagc tttgttgtag gtggaccagt tggtgatttt gaacttttgc
     2401 tttgccacgg aacggtctgc gttgtcggga agatgcgtga tctgatcctt caactcagca
     2461 aaagttcgat ttattcaaca aagccacgtt gtgtctcaaa atctctgatg ttacattgca
     2521 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     2581 aggggtgttt actagaggtt gatcgggcac gtaagaggtt ccaactttca ccataatgaa
     2641 ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct
     2701 aaaatggaga aaaaaatcac gggatatacc accgttgata tatcccaatg gcatcgtaaa
     2761 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
     2821 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     2881 attcacattc ttgcccgcct gatgaacgct cacccggagt ttcgtatggc catgaaagac
     2941 ggtgagctgg tgatctggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     3001 gaaacgtttt cgtccctctg gagtgaatac cacgacgatt tccggcagtt tctccacata
     3061 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     3121 gagaatatgt tttttgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     3181 gtggccaata tggacaactt cttcgccccc gttttcacga tgggcaaata ttatacgcaa
     3241 ggcgacaagg tgctgatgcc gctggcgatc caggttcatc atgccgtttg tgatggcttc
     3301 catgtcggcc gcatgcttaa tgaattacaa cagtactgtg atgagtggca gggcggggcg
     3361 taataatact agctccggca aaaaaacggg caaggtgtca ccaccctgcc ctttttcttt
     3421 aaaaccgaaa agattacttc gcgtttgcca cctgacgtct aagaaaagga atattcagca
     3481 atttgcccgt gccgaagaaa ggcccacccg tgaaggtgag ccagtgagtt gattgctacg
     3541 taattagtta gttagccctt agtgactcga attcgcggcc gcttctagag ttatgacaac
     3601 ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct cgggctggcc
     3661 ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga
     3721 ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt
     3781 tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag atgtgacaga
     3841 cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc
     3901 aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg tggatggagc
     3961 gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt tatcgccagc
     4021 agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa caggtcgctg
     4081 aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa tattgacggc
     4141 cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg gtgataccat
     4201 tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa cagcaaaata
     4261 tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg accgcgaatg
     4321 gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa atcgagataa
     4381 ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga gtatcccggc
     4441 agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt cagagaagaa
     4501 accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg gctcttctcg
     4561 ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg gaccaaagcc
     4621 atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattgattat
     4681 ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct
     4741 acctgacgct ttttatcgca actctctact gtttctccat acccgttttt ttgggctagc
//
