LOCUS       sequence-118470-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses malE-poly-RGD in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pMAL-c5X-poly-RGD
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..38
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             717..734
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      750..821
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 acaataacaa taacaacaac ctcgggatcg aaggcaggtc ttccggtggt tcaggttcgg
       61 gtagtgggcg cggagattca ggtagcggta gcgggtctgg gcgtggtgat tccggtagtg
      121 gttcggggag tggcagggga gacagcggaa gtgggtcggg atctggacgg ggagatagcg
      181 gctcaggcag tgggtctgga aggggagatt ctggttctgg aagtggctca ggaagagggg
      241 actccgggtc aggctcaggt agtggacgag gggatagtgg gagtgggtcc gggagtggaa
      301 gaggtgattc gggaagtgga tctgggagcg gacgtggaga ctcaggatca ggctctggat
      361 cagggcgggg tgactcaggt tccggatctg gcagcggtcg cggtgattca ggaagtggtt
      421 caggatctgg taggggtgat tctgggtcag gttcagggag tggtcgaggt gatagtggta
      481 gtggctccgg ttcaggccgt ggcgacagtg gctcgggatc gggatcagga cgcggcgata
      541 gtggaagcgg ttctggctct gggagaggcg actcgggctc tggttccggc tctggccgag
      601 gtgactctgg tagcggatca ggttctggga ggggcgattc tggatcgggg tcagggtctg
      661 gtcgtgggga ttcagggtca ggatcgggtt ctggtagagg tgacagtggc tcgggacatc
      721 atcaccacca tcattaataa tgaaagcttc aaataaaacg aaaggctcag tcgaaagact
      781 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctga
//
