LOCUS       sequence-118471-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses malE-poly-RGD in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pMAL-c5X-poly-RGD
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(188..278)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(306..333)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(425..511)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(527..544)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
       61 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
      121 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
      181 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
      241 gcggatacat atttgaatgt atttagaaaa ataaacaacg gaccgaaaga gtttgtagaa
      301 acgcaaaaag gccatccgtc aggatggcct tctgcttaat ttgatgcctg gcagtttatg
      361 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcgc aacgttcaaa tccgctcccg
      421 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
      481 agtctttcga ctgagccttt cgttttattt gaagctttca ttattaatga tggtggtgat
      541 gatgtcccga gccactgtca cctctaccag aacccgatcc tgaccctgaa tccccacgac
      601 cagaccctga ccccgatcca gaatcgcccc tcccagaacc tgatccgcta ccagagtcac
      661 ctcggccaga gccggaacca gagcccgagt cgcctctccc agagccagaa ccgcttccac
      721 tatcgccgcg tcctgatccc gatcccgagc cactgtcgcc acggcctgaa ccggagccac
//
