LOCUS       sequence-118474-         491 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses malE-poly(alanylglycine) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pMAL-c5X-poly(alanylglycine)
FEATURES             Location/Qualifiers
     source          1..491
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..35
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             321..338
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      354..440
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ataacaataa caacaacctc gggatcgaag gcaggtcttc cggtgcagga gctggagcag
       61 ggccagaagg ggcaggagcg ggggccggtc ctgaaggtgc tggagccggt gcaggacccg
      121 agggagcagg cgccggagca ggtcccgaag gtgcgggcgc gggtgcaggt cctgagggtg
      181 ctggcgctgg agctgggcct gaaggcgcag gagcaggagc cggaccagaa ggtgccgggg
      241 ctggtgctgg tccagagggg gcgggagctg gtgcaggccc ggaaggtgca ggggctgggg
      301 cgggaccaga gggctcggga catcatcacc accatcatta ataatgaaag cttcaaataa
      361 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      421 ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg
      481 gagggtggcg g
//
