LOCUS       sequence-118482-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express FLAG-His6-tagged dominant active let60, in which Glycine 12 
            is mutated to Valine, in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Iwasa H, Kuroyanagi H, Maimaiti S, Ikeda M, Nakagawa K, Hata Y
  TITLE     Characterization of RSF-1, the Caenorhabditis elegans homolog of the
            Ras-association domain family protein 1.
  JOURNAL   Exp Cell Res. 2013 Feb 1;319(3):1-11. doi: 
            10.1016/j.yexcr.2012.10.008. Epub 2012 Oct 26.
  PUBMED    23103556
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.yexcr.2012.10.008"; journalName: "Exp Cell Res"; date: 
            "2013-02-1- 1"; volume: "319"; issue: "3"; pages: "1-11"
COMMENT     Sequence Label: pClneoFH-let60 G12V
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          151..283
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     266..285
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        328..346
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             362..385
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             392..409
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             416..439
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accgtcagat cactagaagc tttattgcgg tagtttatca cagttaaatt gctaacgcag
       61 tcagtgcttc tgacacaaca gtctcgaact taagctgcag tgactctctt aaggtagcct
      121 tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa
      181 ggagaccaat agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca
      241 cctattggtc ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc
      301 aattacagct cttaaggcta gagtacttaa tacgactcac tataggctag ccggggccat
      361 ggactacaaa gacgatgacg acaaggaatt gcaccatcat catcatcatg aattggacta
      421 caaagacgat gacgacaagg aattcatgac ggagtacaag cttgtggtag ttggagatgt
      481 aggagttggt aaatcagcac tcaccattca actcatccag aatcactttg tcgaagaata
      541 cgacccgacc atagaggaca gctacagaaa gcaggttgtg atagacggcg agacatgcct
      601 cctcgacata ttggataccg ccggacaaga agaatattcg gcgatgcgtg atcagtacat
      661 gaggacaggc gaaggatttc tgttggtttt cgccgtcaac gaggctaaat ctttcgagaa
      721 tgtcgctaac taccgcgagc agattcggag ggtaaaggat tcagatgatg ttcctatggt
      781 cttggttggg aataaatgtg atttgtcatc tcgatcagtc gacttccgaa cagtcagtga
      841 gacagcaaag ggttacggta ttccgaatgt cgacacatct gccaaa
//
