LOCUS       sequence-118485-         534 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses malE-Dros16 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pMAL-c5X-Dros16
FEATURES             Location/Qualifiers
     source          1..534
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(132..222)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(250..277)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(369..455)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(471..488)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
       61 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
      121 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
      181 atgagcggat acatatttga atgtatttag aaaaataaac aacggaccga aagagtttgt
      241 agaaacgcaa aaaggccatc cgtcaggatg gccttctgct taatttgatg cctggcagtt
      301 tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcgcaacgtt caaatccgct
      361 cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag
      421 gcccagtctt tcgactgagc ctttcgtttt atttgaagct ttcattatta atgatggtgg
      481 tgatgatgtc ccgagccata agtatcactt ggtctaccac cgtttcctcc ccca
//
