LOCUS       sequence-118486-         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses malE-Dros16 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pMAL-c5X-Dros16
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..35
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             774..791
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      807..893
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ataacaataa caacaacctc gggatcgaag gcaggtcttc cggtggagct cccggtggcg
       61 gcaatggagg aaggccatca gacacgtatg gagcgcctgg agggggaaat ggcggtagac
      121 cgagtgatac gtatggtgct cctggcgggg gtaatggagg tcgtccatct gatacatacg
      181 gcgcaccagg gggtgggaac ggaggtagac cctcagatac ctatggagcc ccgggtggag
      241 ggaatggtgg ccgtccctct gatacttatg gcgcccccgg cggaggcaac gggggaagac
      301 cgtcagatac ttatggtgcc ccaggtggag gaaatggggg tagaccttct gacacttacg
      361 gtgctccggg aggtggtaat gggggcaggc ccagtgatac ttacggagcc cctggaggtg
      421 gcaatggtgg tcggcctagt gatacctacg gggcaccggg cggtggaaat ggtggaagac
      481 catcggacac atacggtgca cccggaggtg gaaacggagg gaggccaagc gatacttatg
      541 gagcacctgg aggcggaaat ggagggcgtc cttcagatac gtacggagct ccaggaggtg
      601 ggaatggagg cagacctagt gacacctatg gtgcacctgg tggaggtaat ggtgggcgac
      661 cttcggatac ttatggggct cctggtggtg gtaacggagg aagacccagc gacacttatg
      721 gagctcctgg gggaggaaac ggtggtagac caagtgatac ttatggctcg ggacatcatc
      781 accaccatca ttaataatga aagcttcaaa taaaacgaaa ggctcagtcg aaagactggg
      841 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aat
//
