LOCUS       sequence-118487-         582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses malE-AB12 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pMAL-c5X-AB12
FEATURES             Location/Qualifiers
     source          1..582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..39
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             415..432
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      447..533
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaataaca ataacaacaa cctcgggatc gagggaaggt cttccggttt cggcggtatg
       61 ggtggcggta atgctgggtt tgggggaatg ggtggaggca aagcaggttt tggaggcatg
      121 ggcggcggaa atgccggatt cggtggcatg ggtggtggga acgcgggctt cggtggaatg
      181 ggtgggggca aggcagggtt cggtggtatg ggtggtggta acgccggttt tggtgggatg
      241 ggtggtggaa acgctggatt tggcgggatg ggtgggggaa aggctggttt cggtgggatg
      301 ggcggtggta atgcgggttt tggcggaatg ggtggtggca atgcaggatt tggtggtatg
      361 ggcggtggaa aagctggctt tggtggaatg ggcgggggca acgcaggctc gggacatcat
      421 caccaccatc attaataatg aagcttcaaa taaaacgaaa ggctcagtcg aaagactggg
      481 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg
      541 gagcggattt gaacgttgcg aagcaacggc ccggagggtg gc
//
