LOCUS       sequence-11849-e        1211 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1211)
  TITLE     pCMV-Flag-hMcl-1
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMV-Flag-hMcl-1(T163A)
FEATURES             Location/Qualifiers
     source          1..1211
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        34..52
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      87..96
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             96..119
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgtcagatcc gctagcgatt acgccaagct cgaaattaac cctcactaaa gggaacaaaa
       61 gctggagctc caccgcggtg gcggccgcca ccatggatta caaggatgac gacgataagg
      121 cccgggcgga tccactagtc cagtgtggtg gaattgccct tatgtttggc ctcaaaagaa
      181 acgcggtaat cggactcaac ctctactgtg ggggggccgg cttgggggcc ggcagcggcg
      241 gcgccacccg cccgggaggg cgacttttgg ctacggagaa ggaggcctcg gcccggcgag
      301 agataggggg aggggaggcc ggcgcggtga ttggcggaag cgccggcgca agccccccgt
      361 ccaccctcac gccagactcc cggagggtcg cgcggccgcc gcccattggc gccgaggtcc
      421 ccgacgtcac cgcgaccccc gcgaggctgc ttttcttcgc gcccacccgc cgcgcggcgc
      481 cgcttgagga gatggaagcc ccggccgctg acgccatcat gtcgcccgaa gaggagctgg
      541 acgggtacga gccggagcct ctcgggaagc ggccggctgt cctgccgctg ctggagttgg
      601 tcggggaatc tggtaataac accagtacgg acgggtcact accctcggcg ccgccgccag
      661 cagaggagga ggacgagttg taccggcagt cgctggagat tatctctcgg taccttcggg
      721 agcaggccac cggcgccaag gacacaaagc caatgggcag gtctggggcc accagcagga
      781 aggcgctgga gaccttacga cgggttgggg atggcgtgca gcgcaaccac gagacggcct
      841 tccaaggcat gcttcggaaa ctggacatca aaaacgaaga cgatgtgaaa tcgttgtctc
      901 gagtgatgat ccatgttttc agcgacggcg taacaaactg gggcaggatt gtgactctca
      961 tttcttttgg tgcctttgtg gctaaacact tgaagaccat aaaccaagaa agctgcatcg
     1021 aaccattagc agaaagtatc acagacgttc tcgtaaggac aaaacgggac tggctagtta
     1081 aacaaagagg ctgggatggg tttgtggagt tcttccatgt agaggaccta gaaggtggca
     1141 tcaggaatgt gctgctggct tttgcaggtg ttgctggagt aggagctggt ttggcatatc
     1201 taataagatt g
//
