LOCUS       sequence-118491-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression / in vitro transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Feike AC, Rachor K, Gentzel M, Schambony A
  TITLE     Wnt5a/Ror2-induced upregulation of xPAPC requires xShcA.
  JOURNAL   Biochem Biophys Res Commun. 2010 Oct 1;400(4):500-6. doi: 
            10.1016/j.bbrc.2010.08.074. Epub 2010 Aug 21.
  PUBMED    20732301
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2010.08.074"; journalName: "Biochem Biophys Res 
            Commun"; date: "2010-10-1- 1"; volume: "400"; issue: "4"; pages: 
            "500-6"
COMMENT     Sequence Label: pCS2 xRor2-mCherry
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..741)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(309..328)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     578..596
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tacgactcac tatagttcta gaggctcgag ttacttgtac agctcgtcca tgccgccggt
       61 ggagtggcgg ccctcggcgc gttcgtactg ttccacgatg gtgtagtcct cgttgtggga
      121 ggtgatgtcc aacttgatgt tgacgttgta ggcgccgggc agctgcacgg gcttcttggc
      181 cttgtaggtg gtcttgacct cagcgtcgta gtggccgccg tccttcagct tcagcctctg
      241 cttgatctcg cccttcaggg cgccgtcctc ggggtacatc cgctcggagg aggcctccca
      301 gcccatggtc ttcttctgca ttacggggcc gtcggagggg aagttggtgc cgcgcagctt
      361 caccttgtag atgaactcgc cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc
      421 gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg acagcttcaa
      481 gtagtcgggg atgtcggcgg ggtgcttcac gtaggccttg gagccgtaca tgaactgagg
      541 ggacaggatg tcccaggcga agggcagggg gccacccttg gtcaccttca gcttggcggt
      601 ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt
      661 cacggagccc tccatgtgca ccttgaagcg catgaactcc ttgatgatgg ccatgttatc
      721 ctcctcgccc ttgctcacca tgctagcagt ctctgactga atatctgttg catctaattg
      781 agatgtgtca ttgtcaccca gcaattctgt ttctggaaca gaaccttctt ctt
//
