LOCUS       sequence-118495-         682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For sleeping beauty transposition and establishment of an 
            recombinase mediated cassette exchange docking site. To make RMCE in
            cell line instead of flp in cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Callesen MM, Berthelsen MF, Lund S, Fuchtbauer AC, Fuchtbauer EM, 
            Jakobsen JE
  TITLE     Recombinase-Mediated Cassette Exchange (RMCE)-in Reporter Cell Lines
            as an Alternative to the Flp-in System.
  JOURNAL   PLoS One. 2016 Aug 19;11(8):e0161471. doi: 
            10.1371/journal.pone.0161471. eCollection 2016.
  PUBMED    27541869
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-19"; volume: "11(8):e0161471. doi"; pages: " 
            10.1371/journal.pone.0161471. eCollection 2016"
COMMENT     Sequence Label: Transposon-for RMCE_Sleeping
            Beauty_LIR-CAG-Fr...
FEATURES             Location/Qualifiers
     source          1..682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    30..95
                     /label=MCS
                     /note="multiple cloning site"
     CDS             123..182
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     primer_bind     complement(189..208)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gcgacctgcc tagcaaactg gggcacagct ccggactcag atctcgagct caagcttcga
       61 attctgcagt cgacggtacc gcgggcccgg gatccaccgg atctagacct aggggaagcg
      121 gacagtgtac taattatgct ctcttgaaat tggctggaga tgttgagagc aaccctggac
      181 ctaccggtat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
      241 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
      301 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
      361 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
      421 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
      481 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
      541 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
      601 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
      661 ccttcctgga gacctccgcg cc
//
