LOCUS       sequence-118499-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RMCE donor plasmid for FlpO. the marker gene is green fluorescent 
            (mWasabi) with PA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Callesen MM, Berthelsen MF, Lund S, Fuchtbauer AC, Fuchtbauer EM, 
            Jakobsen JE
  TITLE     Recombinase-Mediated Cassette Exchange (RMCE)-in Reporter Cell Lines
            as an Alternative to the Flp-in System.
  JOURNAL   PLoS One. 2016 Aug 19;11(8):e0161471. doi: 
            10.1371/journal.pone.0161471. eCollection 2016.
  PUBMED    27541869
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-19"; volume: "11(8):e0161471. doi"; pages: " 
            10.1371/journal.pone.0161471. eCollection 2016"
COMMENT     Sequence Label: Frt-Atg-Hygro-CMV-green (mWasabi)-PA-F3
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      164..173
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      121 ggtggaattc tgcagatatc cagcacagtg gcggccgctc gaggccacca tggtgagcaa
      181 gggcgaggag accacaatgg gcgtaatcaa gcccgacatg aagatcaagc tgaagatgga
      241 gggcaacgtg aatggccacg ccttcgtgat cgagggcgag ggcgagggca agccctacga
      301 cggcaccaac accatcaacc tggaggtgaa ggagggagcc cccctgccct tctcctacga
      361 cattctgacc accgcgttca gttacggcaa cagggccttc accaagtacc ccgacgacat
      421 ccccaactac ttcaagcagt ccttccccga gggctactct tgggagcgca ccatgacctt
      481 cgaggacaag ggcatcgtga aggtgaagtc cgacatctcc atggaggagg actccttcat
      541 ctacgagata cacctcaagg gcgagaactt cccccccaac ggccccgtga tgcagaagga
      601 gaccaccggc tgggacgcct ccaccgagag gatgtacgtg cgcgacggcg tgctgaaggg
      661 cgacgtcaag atgaagctgc tgctggaggg cggcggccac caccgcgttg acttcaagac
      721 catctacagg gccaagaagg cggtgaagct gcccgactat cactttgtgg accaccgcat
      781 cgagatcctg aaccacgaca aggactacaa caaggtgacc gtttacgaga tcgccgtggc
      841 ccgcaactcc accgacggca tggacg
//
