LOCUS       sequence-11850-e        1206 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1206)
  TITLE     pCMV-Flag-hMcl-1
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMV-Flag-hMcl-1
FEATURES             Location/Qualifiers
     source          1..1206
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        29..47
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      82..91
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             91..114
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gatccgctag cgattacgcc aagctcgaaa ttaaccctca ctaaagggaa caaaagctgg
       61 agctccaccg cggtggcggc cgccaccatg gattacaagg atgacgacga taaggcccgg
      121 gcggatccac tagtccagtg tggtggaatt gcccttatgt ttggcctcaa aagaaacgcg
      181 gtaatcggac tcaacctcta ctgtgggggg gccggcttgg gggccggcag cggcggcgcc
      241 acccgcccgg gagggcgact tttggctacg gagaaggagg cctcggcccg gcgagagata
      301 gggggagggg aggccggcgc ggtgattggc ggaagcgccg gcgcaagccc cccgtccacc
      361 ctcacgccag actcccggag ggtcgcgcgg ccgccgccca ttggcgccga ggtccccgac
      421 gtcaccgcga cccccgcgag gctgcttttc ttcgcgccca cccgccgcgc ggcgccgctt
      481 gaggagatgg aagccccggc cgctgacgcc atcatgtcgc ccgaagagga gctggacggg
      541 tacgagccgg agcctctcgg gaagcggccg gctgtcctgc cgctgctgga gttggtcggg
      601 gaatctggta ataacaccag tacggacggg tcactaccct cgacgccgcc gccagcagag
      661 gaggaggacg agttgtaccg gcagtcgctg gagattatct ctcggtacct tcgggagcag
      721 gccaccggcg ccaaggacac aaagccaatg ggcaggtctg gggccaccag caggaaggcg
      781 ctggagacct tacgacgggt tggggatggc gtgcagcgca accacgagac ggccttccaa
      841 ggcatgcttc ggaaactgga catcaaaaac gaagacgatg tgaaatcgtt gtctcgagtg
      901 atgatccatg ttttcagcga cggcgtaaca aactggggca ggattgtgac tctcatttct
      961 tttggtgcct ttgtggctaa acacttgaag accataaacc aagaaagctg catcgaacca
     1021 ttagcagaaa gtatcacaga cgttctcgta aggacaaaac gggactggct agttaaacaa
     1081 agaggctggg atgggtttgt ggagttcttc catgtagagg acctagaagg tggcatcagg
     1141 aatgtgctgc tggcttttgc aggtgttgct ggagtaggag ctggtttggc atatctaata
     1201 agattg
//
