LOCUS       sequence-118504-         616 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  inducbile color change and cancer regulation: FlpO recombinase 
            dependent expression of KrasG12D cMyc and SV40 large T antigen with 
            doxycycline inducible downregulation through tetKRAB system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Callesen MM, Arnadottir SS, Lyskjaer I, Orntoft MW, Hoyer S, 
            Dagnaes-Hansen F, Liu Y, Li R, Callesen H, Rasmussen MH, Berthelsen 
            MF, Thomsen MK, Schweiger PJ, Jensen KB, Laurberg S, Orntoft TF, 
            Elverlov-Jakobsen JE, Andersen CL
  TITLE     A genetically inducible porcine model of intestinal cancer.
  JOURNAL   Mol Oncol. 2017 Nov;11(11):1616-1629. doi: 10.1002/1878-0261.12136. 
            Epub 2017 Oct 10.
  PUBMED    28881081
REFERENCE   2  (bases 1 to 616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/1878-0261.12136"; journalName: "Mol Oncol"; date: 
            "2017-11"; volume: "11"; issue: "11"; pages: "1616-1629"
COMMENT     Sequence Label: 
            LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-S
            V40LT-IRES-KRABoff-PA-TRE-loxP257-RIR
FEATURES             Location/Qualifiers
     source          1..616
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    24..71
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     regulatory      72..81
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcattttggc aaagattaat taagaagttc ctattccgaa gttcctattc tctagaaagt
       61 ataggaactt cgccaccatg gtgggtgagg atagcgtgct gatcaccgag aacatgcaca
      121 tgaaactgta catggagggc accgtgaacg accaccactt caagtgcaca tccgagggcg
      181 aaggcaagcc ctacgagggc acccagacca tgaagatcaa ggtggtcgag ggcggccctc
      241 tccccttcgc cttcgacatc ctggctacca gcttcatgta cggcagcaaa acctttatca
      301 accacaccca gggcatcccc gacttcttta agcagtcctt ccctgagggc ttcacatggg
      361 agaggatcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc
      421 agaacggctg cctcatctac aacgtcaaga tcaacggggt gaacttccca tccaacggcc
      481 ctgtgatgca gaagaaaaca ctcggctggg aggccagcac cgagatgctg taccccgctg
      541 acagcggcct gagaggccat agccagatgg ccctgaagct cgtgggcggg ggctacctgc
      601 actgctccct caagac
//
