LOCUS       sequence-118510-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Myc-tagged C.elegans EGL-44 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Iwasa H, Maimaiti S, Kuroyanagi H, Kawano S, Inami K, Timalsina S, 
            Ikeda M, Nakagawa K, Hata Y
  TITLE     Yes-associated protein homolog, YAP-1, is involved in the 
            thermotolerance and aging in the nematode Caenorhabditis elegans.
  JOURNAL   Exp Cell Res. 2013 Apr 15;319(7):931-45. doi: 
            10.1016/j.yexcr.2013.01.020. Epub 2013 Feb 7.
  PUBMED    23396260
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.yexcr.2013.01.020"; journalName: "Exp Cell Res"; date: 
            "2013-04-15- 15"; volume: "319"; issue: "7"; pages: "931-45"
COMMENT     Sequence Label: pClneoMyc'-EGL-44
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          151..283
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     266..285
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        328..346
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             367..396
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 accgtcagat cactagaagc tttattgcgg tagtttatca cagttaaatt gctaacgcag
       61 tcagtgcttc tgacacaaca gtctcgaact taagctgcag tgactctctt aaggtagcct
      121 tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa
      181 ggagaccaat agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca
      241 cctattggtc ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc
      301 aattacagct cttaaggcta gagtacttaa tacgactcac tataggctag cagacccccc
      361 aacatggagc agaagcttat cagcgaggag gacctgctcg aggaattcac gcgtatgaac
      421 tcaatgtttt gttcagagtt gcaacaagcg ctgccgcaga tgtcggaaga cgtagcagtc
      481 acggcgcaaa caattctcaa tggaaccccg ccccataatc attttcgaaa agagtggctc
      541 gctggagcag caggaacgac aacgcccacg acgacctctg gcggacaaat gatgacgctc
      601 tcccctcccg ccggtgacgg accaggttcc gctggctcaa tggctcccga atcgacttct
      661 tccctttccg acttgtctgg tgacgcggaa ggtgtgtgga gtatagacat tgatcaagca
      721 tttcaagaag cgttagccat ctatccacct tgtggacgaa ggaaaattat catcagtgac
      781 gaagggaaaa tgtatggaag gaacgaacta atcgctaggt atatcaaatt gcgatgtgga
      841 a
//
