LOCUS       sequence-118516-         617 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses malE-35-H-6 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pMAL-c5X-35-H-6
FEATURES             Location/Qualifiers
     source          1..617
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..29
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             444..461
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      477..563
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ataacaacaa cctcgggatc gaaggcaggt cttccggtgc agcagcgcaa gcagctcagg
       61 ctcaggctgc agcggaagca gccgcccaag ctgcccaggc acaggcggca gcacaggccg
      121 cacaggctca agctgctgcg gaggcagctg cgcaagcggc acaagctcag gcagcggctc
      181 aagccgctca ggcccaagca gctgcagagg ctgctgcaca ggcagctcaa gcacaggctg
      241 ccgcacaagc tgcacaagca caagcagcag cagaagctgc ggcgcaagct gctcaggcac
      301 aagcggctgc ccaagcggcc caggcgcagg cagcagctga agctgcagca caagccgcgc
      361 aagcccaagc cgcagcccag gctgctcaag ctcaagcggc ggccgaagct gccgctcaag
      421 cagcccaagc tcaaggctcg ggacatcatc accaccatca ttaataatga aagcttcaaa
      481 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      541 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc
      601 ccggagggtg gcgggca
//
