LOCUS       sequence-118519-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNA against human CEBPB along with Cas9 with 2A GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: PX458_CEBPB_1
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        121..369
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     121..141
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     292..311
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        389..464
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        561..846
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
       61 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      121 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
      181 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      241 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      301 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      361 cgaaacaccg ctccggccac tgctagcggt tttagagcta gaaatagcaa gttaaaataa
      421 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt gttttagagc
      481 tagaaatagc aagttaaaat aaggctagtc cgtttttagc gcgtgcgcca attctgcaga
      541 caaatggctc tagaggtacc cgttacataa cttacggtaa atggcccgcc tggctgaccg
      601 cccaacgacc cccgcccatt gacgtcaata gtaacgccaa tagggacttt ccattgacgt
      661 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      721 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttgtgcccag
      781 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      841 accatggtcg aggtgagccc
//
