LOCUS       sequence-118527-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Specific and conditional cell ablation of beta cells in the 
            zebrafish pancreas.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Curado S, Anderson RM, Jungblut B, Mumm J, Schroeter E, Stainier DY
  TITLE     Conditional targeted cell ablation in zebrafish: a new tool for 
            regeneration studies.
  JOURNAL   Dev Dyn. 2007 Apr . 236(4):1025-35.
  PUBMED    17326133
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Apr . 236(4):1025-35."
COMMENT     Sequence Label: ins:CFP-NTR
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(111..623,624..626,627..629))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     304..323
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     563..584
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
       61 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg
      121 tggcggatct tgaagttggc cttgatgccg ttcttctgct tgtcggcggt gatatagacg
      181 ttgtggctga tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
      241 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
      301 cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg
      361 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac
      421 tgcacgcccc aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg
      481 gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac
      541 acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg
      601 gtgaacagct cctcgccctt gctcaccatg aattcctgca gcccggggga tccgtcacac
      661 tgacacaaac acacacatac aatctctgtt aatcaaaaac atgtacacaa gctacactcc
      721 gcattctctg tagatttacc atcccgctcg acactcacct gttctcgaag cagaggcgag
      781 gaatggtgga gatggtggtg gagatgtttt ggg
//
