LOCUS       sequence-118532-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses malE-Darpin E_01 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pMAL-c5X-Darpin E_01
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(174..264)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(292..319)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(411..497)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(513..530)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
       61 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
      121 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
      181 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
      241 gaatgtattt agaaaaataa acaacggacc gaaagagttt gtagaaacgc aaaaaggcca
      301 tccgtcagga tggccttctg cttaatttga tgcctggcag tttatggcgg gcgtcctgcc
      361 cgccaccctc cgggccgttg cttcgcaacg ttcaaatccg ctcccggcgg atttgtccta
      421 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga
      481 gcctttcgtt ttatttgaag ctttcattat taatgatggt ggtgatgatg tcccgagccc
      541 tgtaagattt ctgctaggtc ttcgttacca ttgtctatac taatatcaaa agctgtttta
      601 ccgaatttat cttgagcatt cacatcagca ccgtgtttca gtaatacctc tacaatttcg
      661 agatgcccat tatgtgcagc tagatgtagt ggtgtatctc ctatgtaatc actcgcattg
      721 acatccgctc cattctttaa taagacttca actatttcaa ggtgaccatc tgc
//
