LOCUS       sequence-118542-         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses malE-Darpin E_01 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pMAL-c5X-Darpin E_01
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..35
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             513..530
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      546..632
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ataacaataa caacaacctc gggatcgaag gcaggtcttc cggtgatctg ggaaaaaagt
       61 tactagaagc agcaagagct ggacaggatg atgaggttag aatactcatg gcaaacgggg
      121 ccgatgttaa tgcagacgat acttggggat ggacccctct tcatttggct gcctatcagg
      181 gacatttaga gattgtggaa gttctactta aaaatggcgc tgacgtaaac gcttatgact
      241 atattggttg gactccttta cacttagccg cagatggtca ccttgaaata gttgaagtct
      301 tattaaagaa tggagcggat gtcaatgcga gtgattacat aggagataca ccactacatc
      361 tagctgcaca taatgggcat ctcgaaattg tagaggtatt actgaaacac ggtgctgatg
      421 tgaatgctca agataaattc ggtaaaacag cttttgatat tagtatagac aatggtaacg
      481 aagacctagc agaaatctta cagggctcgg gacatcatca ccaccatcat taataatgaa
      541 agcttcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      601 tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga
      661 agcaacggcc cggaggggtg gcgggcagga cgccc
//
