LOCUS       sequence-118543-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses malE-GLP-1 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pMAL-c5X-GLP-1
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..38
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             633..650
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      666..752
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 acaataacaa taacaacaac ctcgggatcg aaggcaggtc ttccggtgga gcacatggag
       61 agggtacatt tacctcagat gttagtagtt atttggaaga acaggccgcc aaagaattca
      121 tagcttggtt agttaaagga agaggtgcac acggggaggg aacttttaca tcggatgtct
      181 caagttatct ggaggaacag gcagcgaaag agttcattgc atggctggta aaaggacgtg
      241 gggctcacgg tgaggggact tttacttcag acgtttcatc atatcttgaa gaacaagcag
      301 caaaggaatt tatagcatgg ttggtcaagg gtcgtggagc tcatggggaa ggaaccttta
      361 ctagtgatgt gtcatcttat ttagaggaac aagcggctaa agagtttata gcgtggctag
      421 tcaaaggtcg cggagcgcac ggagaaggta cttttacgag tgacgtatca tcctatctcg
      481 aagagcaagc tgcgaaggag tttattgctt ggcttgtgaa aggtaggggt gcccatggtg
      541 aagggacatt cacttctgat gtatctagtt acttagaaga gcaggctgca aaagaattta
      601 tcgcctggct cgttaaaggg cgtggctcgg gacatcatca ccaccatcat taataatgaa
      661 agcttcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      721 tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga
      781 agcaacgg
//
