LOCUS       sequence-118545-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses malE-Ad I in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pMAL-c5X-Ad I
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(191..281)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(309..336)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(428..514)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(530..547)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
       61 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
      121 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
      181 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
      241 tgagcggata catatttgaa tgtatttaga aaaataaaca acggaccgaa agagtttgta
      301 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt aatttgatgc ctggcagttt
      361 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cgcaaggttc aaatccgctc
      421 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
      481 cccagtcttt cgactgagcc tttcgtttta tttgaagctt tcattattaa tgatggtggt
      541 gatgatgtcc cgagccctta ccgtcagata ctgttaaggg tgctgatgtt tgcacagaga
      601 gtttgccatc gcttacagtt aatggagctg aggtttgtac cgacaacttt ccgtcactaa
      661 cagtcagtgg ggctgaagtc tgaacactca atttcccgtc cgaaactgtt aggggagcac
      721 tagtctggac agatagcttc ccatcggaaa ccgtgagagg cgctgacgtt tgtacggata
      781 attttccgtc tgacacagtc aacggtgcac ttgtttgtac acttaacttg ccatcactta
      841 cggtcaatgg tgca
//
