LOCUS       sequence-118547-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses malE-Ad I in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pMAL-c5X-Ad I
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..38
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             687..704
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      720..806
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 acaataacaa taacaacaac ctcgggatcg aaggcaggtc ttccggtctt agtgtacaga
       61 catccgcacc tctaactgtc tcggatggta aattatcggt tcaaacgagt gcccctctta
      121 cagtgtctga tggtaagtta tcagtccaaa cttccgctcc gttaactgtg agcgacggca
      181 aattatctgt tcaaaccagt gcgccactaa ctgtttctga tggaaaactt tcagtacaga
      241 cctcggcccc cttgacagtc tccgacggta aacttagcgt tcaaacatct gctccactta
      301 ctgtatcaga tggaaagctc tcagttcaaa cttcggctcc tttaacagtg agtgatggga
      361 aactatcagt acaaacttct gcaccattga ccgtaagtga tggcaagtta agtgtacaaa
      421 caagtgcacc gttgactgtg tcagacggaa aattatccgt acaaacgtca gcgcctctca
      481 cggtttccga tgggaagcta tctgtccaga ctagtgctcc cctaacagtt tcggacggga
      541 aattgagtgt tcagacttca gccccactga ctgttagtga cggaaagttg tcggtacaaa
      601 cctcagctcc attaactgta agcgatggca aactctctgt gcaaacatca gcacccttaa
      661 cagtatctga cggtaagggc tcgggacatc atcaccacca tcattaataa tgaaagcttc
      721 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      781 tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacctt gcgaagcaac
      841 ggcccgga
//
