LOCUS       sequence-118548-         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses malE-MAP in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pMAL-c5X-MAP
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..35
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             534..551
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      567..653
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ataacaataa caacaacctc gggatcgaag gaaggtcttc cggtgctaaa ccgtcttacc
       61 cacctaccta caaagctaaa ccttcctatc cacctactta taaagcgaag ccttcatacc
      121 cgcccacata taaagcaaaa cccagctacc ctccaactta caaagcgaaa cctagctatc
      181 ctccaaccta caaggcaaag ccgagttacc ccccgacgta taaggctaaa ccaagttatc
      241 cgcctacata taaggccaag cccagttatc ctcctaccta taaggcaaaa ccttcgtacc
      301 ctcctacgta caaagccaaa ccctcgtatc caccgaccta taaagccaag ccatcatatc
      361 caccaacgta taaagctaag ccctcatatc ccccaacata caaggccaaa ccatcgtatc
      421 ctcccactta caaggctaag ccgtcatatc ctccgactta taaggcgaaa ccatcttatc
      481 cccctacata caaagcaaag cctagttatc cacccacgta caagggctcg ggacatcatc
      541 accaccatca ttaataatga aagcttcaaa taaaacgaaa ggctcagtcg aaagactggg
      601 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg
      661 gagcggattt gaacgttgcg aagcaacggc ccggaggg
//
