LOCUS       sequence-118550-         599 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses malE-BRT17 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pMAL-c5X-BRT17
FEATURES             Location/Qualifiers
     source          1..599
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(187..277)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(305..332)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(424..510)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(526..543)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
       61 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
      121 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
      181 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
      241 cggatacata tttgaatgta tttagaaaaa taaacaacgg accgaaagag tttgtagaaa
      301 cgcaaaaagg ccatccgtca ggatggcctt ctgcttaatt tgatgcctgg cagtttatgg
      361 cgggcgtcct gcccgccacc ctccgggccg ttgcttcgca acgttcaaat ccgctcccgg
      421 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
      481 gtctttcgac tgagcctttc gtcttatttg aagctttcat tattaatgat ggtggtgatg
      541 atgtcccgag ccgtccggat actgcgccat tgcctcgacc agcttttcga tgcctgcct
//
