LOCUS       sequence-118551-         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses malE-BRT17 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pMAL-c5X-BRT17
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..31
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             713..730
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 caataacaac aacctcggga tcgaaggcag gtcttccggt ggtggagcag gtaacgacac
       61 attatatgga ggcgcaggta atgatacttt gtacggtgga gcgggcaacg atacgcttta
      121 tggcggtgcc ggaaatgata cattatacgg gggcgcggga aatgacaccc tttatggagg
      181 ggccgggaac gataccttat atggcggagc tggtaatgat acgttatatg gtggagccgg
      241 taatgataca ctatacggtg gtgcaggaaa tgatacttta tatgggggag caggcaatga
      301 tactctttat ggtggtgctg gtaacgatac tctctacgga ggtgcgggta atgacacact
      361 ttacggcggg gcagggaatg acactctgta tgggggtgct gggaatgata ccttgtatgg
      421 tggcgctggc aatgacacgt tgtatggagg tgctggaaat gataccctct atggtggggc
      481 tggaaacgat acattgtata tcaacgcggg ggcggaccag ctgtggttcg cgcgccaggg
      541 caacgacctg gagatccgca ttctcggcac cgacgatgca cttaccgtgc acgactggta
      601 tcgcgacgcc gatcaccggg tggaaatcat ccatgccgcc aaccaggcgg tagaccaggc
      661 aggcatcgaa aagctggtcg aggcaatggc gcagtatccg gacggctcgg gacatcatca
      721 ccaccatcat taataatgaa agcttcaaat aagacgaaag gctcagtcga aa
//
